外源 K-IBA对北美香柏扦插生根和内源激素含量变化的影响
王义强 123王启业 2华连滩 2彭牡丹 2钟洁 2罗浪 2

1. 中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙 410004; 2. 中南林业科技大学生物技术实验室,湖南长沙 410004; 3. 国家林业局生物乙醇研究中心,湖南长沙 410004

北美香柏;扦插;不定根发育;内源激素

Effects of K-IBA treatments on adventitious rooting and endogenous hormones contents of shoot cuttings of Thuja occidentals L.
WANG Yiqiang1,2,3, WANG Qiye2, HUA Liantan2, PENG Mudan2, ZHONG Jie2, LUO Lang2

1. Key Lab of Non-wood Forest Nurturing and Protection of National Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, Hunan, China; 2. Biotechnology Laboratory of Central South University of Forestry and Technology, Changsha 410004, Hunan, China; 3.Bio-ethanol Research Center of State Forestry Administration, Changsha 410004, Hunan, China

shoot cuttings; rooting; plant growth regulator; endogenous hormones

DOI: 10.14067/j.cnki.1673-923x.2017.10.002

备注

以北美香柏 Thuja occidentals L. 2年生枝条为材料,采用 3-吲哚丁酸钾盐(K-IBA)3个浓度(3 000、 6 000、10 000 mg/L)处理扦插生根试验,清水处理为对照。扦插 60天后统计插穗生根率、平均根长、最长根长、平均生根数。扦插后每隔 15 d从 6 000 mg/L K-IBA与对照处理插穗的基部取样,测定生根过程中内源激素吲哚乙酸( IAA)、赤霉素 (GA)、脱落酸( ABA)和玉米素( ZT)含量的变化。结果 表明:外源 K-IBA对北美香柏扦插生根有促进作用,其中 6 000 mg/L K-IBA的处理效果最好,其生根率为 87.78%、生根数 22.33根、平均根长 12.33 mm、最长根长 75.20 mm,显著高于对照。与对照相比, 6 000 mg/L K-IBA处理组的内源 IAA含量、 GA 含量提高,而内源 ABA、ZT含量下降,促使生根率提高。在北美香柏扦插生根过程中, K-IBA处理调节了内源激素 IAA、GA、ABA和 ZT含量的变化,而内源激素的动态变化,共同调控插穗不定根的形成。

The shoot cuttings of Thuja occidentals L. were collected and treated with indole-3-butyric acid (K-IBA) (concentrations 3 000, 6 000 and 10 000 mg/L), Water treatment as a control. The rooting rate, average root length, the longest root length and average rooting number were recorded after cutting for 60 days; The endogenous hormones(IAA, GA,ABA, ZT) contents of cuttings of 6 000 mg/L K-IBA and control treatment were detected every 15 days interval.The significant difference was found among the four treatments. K-IBA promoted the rooting of Thuja occidentals L, Among them, 6 000 mg/L K-IBA has the best treatment effect, which was significantly higher than that of CK. The rooting rate was 87.78%, the number of rooting was 22.33, the average root length was 12.33 mm, the longest root length was 75.20 mm. Compared with the control, the content of endogenous IAA and GA increased in 6 000 mg/L K-IBA treatment group, while the contents of endogenous ABA and ZT decreased, then promoted the rooting. K-IBA regulates the changes of endogenous hormones IAA, GA, ABA and ZT, and the dynamic changes of endogenous hormones regulate the formation of adventitious roots in the process of rooting.