不同倍性滇杨叶片解剖结构差异分析
邱 芬1，2，辛亚龙1，2，唐军荣1，2，李 斌1，2，何承忠1，2，许 程2，辛培尧1，2

1. 西南林业大学 国家林业局西南地区生物多样性保育重点实验室，云南 昆明 650224；2. 西南林业大学 云南省高校林木遗传改良与繁育重点实验室，云南 昆明 650224

滇杨;多倍体;解剖结构;差异

Analysis of leaf anatomical structure differences in Populus yunnanensis with different ploidy
QIU Fen1,2, XIN Yalong1,2, TANG Junrong1,2, LI Bin1,2, HE Chengzhong1,2, XU Cheng2, XIN Peiyao1,2

1.Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry Administration, Southwest Forestry University,Kunming 650224, Yunnan, China; 2. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, Yunnan, China

Populus yunnanensis; polyploid; anatomicale structure; differences

DOI: 10.14067/j.cnki.1673-923x.2017.06.009

备注

以前期诱导获得的5 株多倍体滇杨一年生无性系植株为材料，二倍体滇杨为对照，对其叶片进行石蜡包埋、切片，观察其解剖结构差异，测定叶片厚度、栅栏组织厚度、海绵组织厚度，分析多倍化后滇杨叶片的变异特性。结果 表明：不同倍性滇杨在叶片解剖结构各性状间均存在不同表现。不同个体间叶片厚度、CTR、SR、上表皮相对厚度、下表皮相对厚度间均存在显著差异，滇杨经多倍化处理后能引起栅栏组织、海绵组织的变化，表现为基因的剂量效应差异。同时，不同倍性滇杨叶片CTR、SR 的变化并不与滇杨的倍性成正相关趋势，这为滇杨遗传改良提供了选择的物质基础。研究结果可为滇杨的抗性育种相关性状的早期选择提供理论依据。

Leaves from annual clone individual plant of five polyploid Populus yunnanensis and a diploid plant, as control, were usedto embed with paraffin and slice, in order to observe the leaves anatomical structure. The leaf thickness, palisade tissue thickness andspongy tissue thickness were measured to analyze the difference of P.yunnanensis with different ploidy. The results showed that differentploidy P.yunnanensis had the different representation on traits of the leaf anatomical structure. There were significant differences in leafthickness, CTR, SR, relative thickness of epicuticle, and relative thickness of lower epidermis of P.yunnanensis in different individuals,which indicated that polyploidy could make the palisade tissue and spongy tissue changed, because of gene dose effect. At the sametime. The changes on CTR and SR in leaves of P.yunnanensis were not positive correlation with the their ploidy, which offered theselective material basis of P.yunnanensis breeding. Theoretical basis on the early selection of P.yunnanensis resistance breeding could beprovided from these results.